Instruct PartnerPlease find below the program of the next FRISBI/ReNaFoBis webinar on May 15th at 01:00 PM Paris
Speaker: Marc Jamin, IBS, Grenoble, France
Tittle: Deciphering the mechanisms of liquid phase separation induced by rabies virus phosphoprotein
Khadeeja Mubashira, Fella Bouchama, Caroline Mas, Christine Ebel, Sylvain Prevost, Thomas Zemb, and Marc Jamin,
Rabies virus (RABV) generates membrane-less liquid organelles (Negri bodies) in the cytoplasm of its host cell, where genome transcription and replication and nucleocapsid assembly take place, but the mechanisms of their assembly and maturation remain to be explained. An essential component of the viral RNA synthesizing machine, the phosphoprotein (P), acts as a scaffold protein for the assembly of these condensates. This intrinsically disordered protein forms star-shaped dimers with N-terminal negatively charged flexible arms and C-terminal globular domains exhibiting a large dipole moment. Our study shows that in vitro self-association of RABV P drives a complex thermoresponsive phase separation with a lower critical solution temperature. Protein dimers assemble already below the saturation concentration, and condensation is driven by attractive conformation-specific interactions leading to reentrant liquid phase separation over a narrow range of salt concentration. We propose a minimal molecular model in which P can adopt three limit conformational states and the disordered N-terminal arms control the interactions between giant dipoles that is consistent with our observations.
e-mail: marc.jamin@ibs.fr
PLEASE REGISTER HERE:
https://us06web.zoom.us/webinar/register/WN_hx7hikloSpexd40swI_UnQ
Marie-Christine for the organizing committee
